10x-ATAC-RNA-MULTI/Illumina

• https://doi.org/10.1038/s41592-019-0433-8
• Single Cell Multiome ATAC + Gene Expression + MULTI-seq
• rna, atac, tag

Final library

10x-ATAC-RNA-MULTI-seq RNA

AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNXAGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNNNATCTCGTATGCCGTCTTCTGCTTG

1. RNA-illumina_p5

   • region_type: illumina_p5
   • sequence_type: fixed
   • sequence:

     AATGATACGGCGACCACCGAGATCTACAC

   • min_len: 29
   • max_len: 29
   • onlist: None
   • regions:
2. RNA-truseq_read1

   • region_type: truseq_read1
   • sequence_type: fixed
   • sequence:

     TCTTTCCCTACACGACGCTCTTCCGATCT

   • min_len: 29
   • max_len: 29
   • onlist: None
   • regions:
3. RNA-R1.fastq.gz

   • region_type: fastq
   • sequence_type: joined
   • sequence:

     NNNNNNNNNNNNNNNNNNNNNNNNNNNN

   • min_len: 28
   • max_len: 28
   • onlist: None
   • regions:
     1. RNA-cell_barcode

        • region_type: barcode
        • sequence_type: onlist
        • sequence:

          NNNNNNNNNNNNNNNN

        • min_len: 16
        • max_len: 16
        • onlist: 737K-august-2016.txt (md5: f62a276e262fdd85262a889d0f48556b)
        • regions:
     2. RNA-umi

        • region_type: umi
        • sequence_type: random
        • sequence:

          NNNNNNNNNNNN

        • min_len: 12
        • max_len: 12
        • onlist: None
        • regions:
4. RNA-R2.fastq.gz

   • region_type: fastq
   • sequence_type: joined
   • sequence:

     X

   • min_len: 1
   • max_len: 98
   • onlist: None
   • regions:
     1. RNA-cDNA

        • region_type: cdna
        • sequence_type: random
        • sequence:

          X

        • min_len: 1
        • max_len: 98
        • onlist: None
        • regions:
5. RNA-truseq_read2

   • region_type: truseq_read2
   • sequence_type: fixed
   • sequence:

     AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC

   • min_len: 34
   • max_len: 34
   • onlist: None
   • regions:
6. RNA-I1.fastq.gz

   • region_type: fastq
   • sequence_type: joined
   • sequence:

     NNNNNNNN

   • min_len: 8
   • max_len: 8
   • onlist: None
   • regions:
     1. RNA-index7

        • region_type: index7
        • sequence_type: onlist
        • sequence:

          NNNNNNNN

        • min_len: 8
        • max_len: 8
        • onlist: index7_onlist_RNA-ATAC.txt (md5: fcb5fe7d14ffc9984cd5450413c7a742)
        • regions:
7. RNA-illumina_p7

   • region_type: illumina_p7
   • sequence_type: fixed
   • sequence:

     ATCTCGTATGCCGTCTTCTGCTTG

   • min_len: 24
   • max_len: 24
   • onlist: None
   • regions:

ATAC

AATGATACGGCGACCACCGAGATCTACACNNNNNNNNNNNNNNNNCGCGTCTGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAGXXCTGTCTCTTATACACATCTCCGAGCCCACGAGACNNNNNNNNATCTCGTATGCCGTCTTCTGCTTG

1. ATAC-illumina_p5

   • region_type: illumina_p5
   • sequence_type: fixed
   • sequence:

     AATGATACGGCGACCACCGAGATCTACAC

   • min_len: 29
   • max_len: 29
   • onlist: None
   • regions:
2. ATAC-I2.fastq.gz

   • region_type: fastq
   • sequence_type: joined
   • sequence:

     NNNNNNNNNNNNNNNN

   • min_len: 16
   • max_len: 16
   • onlist: None
   • regions:
     1. ATAC-cell_barcode

        • region_type: barcode
        • sequence_type: onlist
        • sequence:

          NNNNNNNNNNNNNNNN

        • min_len: 16
        • max_len: 16
        • onlist: 737K-cratac-v1.txt (md5: ab645450cf30cc51077f6c030b665297)
        • regions:
3. ATAC-spacer

   • region_type: fastq
   • sequence_type: fixed
   • sequence:

     CGCGTCTG

   • min_len: 8
   • max_len: 8
   • onlist: None
   • regions:
4. ATAC-nextera_read1

   • region_type: nextera_read1
   • sequence_type: joined
   • sequence:

     TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG

   • min_len: 33
   • max_len: 33
   • onlist: None
   • regions:
     1. s5

        • region_type: s5
        • sequence_type: fixed
        • sequence:

          TCGTCGGCAGCGTC

        • min_len: 14
        • max_len: 14
        • onlist: None
        • regions:
     2. ME1

        • region_type: ME1
        • sequence_type: fixed
        • sequence:

          AGATGTGTATAAGAGACAG

        • min_len: 19
        • max_len: 19
        • onlist: None
        • regions:
5. ATAC-R1.fastq.gz

   • region_type: fastq
   • sequence_type: joined
   • sequence:

     X

   • min_len: 1
   • max_len: 98
   • onlist: None
   • regions:
     1. gDNA-1

        • region_type: gdna
        • sequence_type: random
        • sequence:

          X

        • min_len: 1
        • max_len: 98
        • onlist: None
        • regions:
6. ATAC-R2.fastq.gz

   • region_type: gdna
   • sequence_type: joined
   • sequence:

     X

   • min_len: 1
   • max_len: 98
   • onlist: None
   • regions:
     1. gDNA-2

        • region_type: gdna
        • sequence_type: random
        • sequence:

          X

        • min_len: 1
        • max_len: 98
        • onlist: None
        • regions:
7. ATAC-nextera_read2

   • region_type: nextera_read2
   • sequence_type: joined
   • sequence:

     CTGTCTCTTATACACATCTCCGAGCCCACGAGAC

   • min_len: 34
   • max_len: 34
   • onlist: None
   • regions:
     1. ME2

        • region_type: ME2
        • sequence_type: fixed
        • sequence:

          CTGTCTCTTATACACATCT

        • min_len: 19
        • max_len: 19
        • onlist: None
        • regions:
     2. s7

        • region_type: s7
        • sequence_type: fixed
        • sequence:

          CCGAGCCCACGAGAC

        • min_len: 15
        • max_len: 15
        • onlist: None
        • regions:
8. ATAC-I1.fastq.gz

   • region_type: fastq
   • sequence_type: joined
   • sequence:

     NNNNNNNN

   • min_len: 8
   • max_len: 8
   • onlist: None
   • regions:
     1. ATAC-index7

        • region_type: index7
        • sequence_type: onlist
        • sequence:

          NNNNNNNN

        • min_len: 8
        • max_len: 8
        • onlist: index7_onlist_RNA-ATAC.txt (md5: fcb5fe7d14ffc9984cd5450413c7a742)
        • regions:
9. ATAC-illumina_p7

   • region_type: illumina_p7
   • sequence_type: fixed
   • sequence:

     ATCTCGTATGCCGTCTTCTGCTTG

   • min_len: 24
   • max_len: 24
   • onlist: None
   • regions:

MULTI sample barcode

AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACNNNNNNNNATCTCGTATGCCGTCTTCTGCTTG

1. MULTI-illumina_p5

   • region_type: illumina_p5
   • sequence_type: fixed
   • sequence:

     AATGATACGGCGACCACCGAGATCTACAC

   • min_len: 29
   • max_len: 29
   • onlist: None
   • regions:
2. MULTI-truseq_read1

   • region_type: truseq_read1
   • sequence_type: fixed
   • sequence:

     TCTTTCCCTACACGACGCTCTTCCGATCT

   • min_len: 29
   • max_len: 29
   • onlist: None
   • regions:
3. MULTI-R1.fastq.gz

   • region_type: fastq
   • sequence_type: joined
   • sequence:

     NNNNNNNNNNNNNNNNNNNNNNNNNNNN

   • min_len: 28
   • max_len: 28
   • onlist: None
   • regions:
     1. barcode

        • region_type: barcode
        • sequence_type: onlist
        • sequence:

          NNNNNNNNNNNNNNNN

        • min_len: 16
        • max_len: 16
        • onlist: 737K-august-2016.txt (md5: f62a276e262fdd85262a889d0f48556b)
        • regions:
     2. umi

        • region_type: umi
        • sequence_type: random
        • sequence:

          NNNNNNNNNNNN

        • min_len: 12
        • max_len: 12
        • onlist: None
        • regions:
4. MULTI-R2.fastq.gz

   • region_type: fastq
   • sequence_type: joined
   • sequence:

     NNNNNNNN

   • min_len: 8
   • max_len: 8
   • onlist: None
   • regions:
     1. MULTI-seq barcode

        • region_type: barcode
        • sequence_type: onlist
        • sequence:

          NNNNNNNN

        • min_len: 8
        • max_len: 8
        • onlist: MULTIbc_onlist.txt (md5: 66d841ec290d6c93e4b02638f97f3c23)
        • regions:
5. MULTI-smallrna truseq_read2

   • region_type: truseq_read2
   • sequence_type: fixed
   • sequence:

     TGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC

   • min_len: 33
   • max_len: 33
   • onlist: None
   • regions:
6. MULTI-I1.fastq.gz

   • region_type: fastq
   • sequence_type: joined
   • sequence:

     NNNNNNNN

   • min_len: 8
   • max_len: 8
   • onlist: None
   • regions:
     1. MULTI-index7

        • region_type: index7
        • sequence_type: onlist
        • sequence:

          NNNNNNNN

        • min_len: 8
        • max_len: 8
        • onlist: index7_onlist_MULTI.txt (md5: dea9fa960eb2f11b18d47f978c596a79)
        • regions:
7. MULTI-illumina_p7

   • region_type: illumina_p7
   • sequence_type: fixed
   • sequence:

     ATCTCGTATGCCGTCTTCTGCTTG

   • min_len: 24
   • max_len: 24
   • onlist: None
   • regions: