CEL-Seq

• https://doi.org/10.1016/j.celrep.2012.08.003
• barcoding and pooling samples before amplifying RNA with in vitro transcription
• rna

Final library

rna

AATGATACGGCGACCACCGAGATCTACACGTTCAGAGTTCTACAGTCCGACGATCNNNNNNNNXXTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACNNNNNNATCTCGTATGCCGTCTTCTGCTTG

1. illumina_p5

   • region_type: illumina_p5
   • sequence_type: fixed
   • sequence:

     AATGATACGGCGACCACCGAGATCTACAC

   • min_len: 29
   • max_len: 29
   • onlist: None
   • regions:
2. RA5

   • region_type: linker
   • sequence_type: fixed
   • sequence:

     GTTCAGAGTTCTACAGTCCGACGATC

   • min_len: 26
   • max_len: 26
   • onlist: None
   • regions:
3. cell_bc

   • region_type: barcode
   • sequence_type: random
   • sequence:

     NNNNNNNN

   • min_len: 8
   • max_len: 8
   • onlist: None
   • regions:
4. poly_T

   • region_type: poly_T
   • sequence_type: random
   • sequence:

     X

   • min_len: 1
   • max_len: 98
   • onlist: None
   • regions:
5. cDNA

   • region_type: cdna
   • sequence_type: random
   • sequence:

     X

   • min_len: 1
   • max_len: 98
   • onlist: None
   • regions:
6. RA3

   • region_type: linker
   • sequence_type: fixed
   • sequence:

     TGGAATTCTCGGGTGCCAAGG

   • min_len: 21
   • max_len: 21
   • onlist: None
   • regions:
7. link_1

   • region_type: linker
   • sequence_type: fixed
   • sequence:

     AACTCCAGTCAC

   • min_len: 12
   • max_len: 12
   • onlist: None
   • regions:
8. sample_bc

   • region_type: index7
   • sequence_type: onlist
   • sequence:

     NNNNNN

   • min_len: 6
   • max_len: 6
   • onlist: sample_bc_onlist.txt (md5: None)
   • regions:
9. illumina_p7

   • region_type: illumina_p7
   • sequence_type: fixed
   • sequence:

     ATCTCGTATGCCGTCTTCTGCTTG

   • min_len: 24
   • max_len: 24
   • onlist: None
   • regions: