Microwell-seq

• https://doi.org/10.1016/j.cell.2018.02.001
• a high-throughput and low-cost scRNA-seq platform using simple, inexpensive devices
• RNA

Final library

RNA

AATGATACGGCGACCACCGAGATCTACACGCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTACGTNNNNNNCGACTCACTACAGGGNNNNNNTCGGTGACACGATCGNNNNNNNNNNNNXXCTGTCTCTTATACACATCTCCGAGCCCACGAGACNNNNNNNNATCTCGTATGCCGTCTTCTGCTTG

1. illumina_p5

   • region_type: illumina_p5
   • sequence_type: fixed
   • sequence:

     AATGATACGGCGACCACCGAGATCTACAC

   • min_len: 29
   • max_len: 29
   • onlist: None
   • regions:
2. read_1_primer

   • region_type: read_1_primer
   • sequence_type: fixed
   • sequence:

     GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTACGT

   • min_len: 39
   • max_len: 39
   • onlist: None
   • regions:
3. bc1

   • region_type: bc1
   • sequence_type: onlist
   • sequence:

     NNNNNN

   • min_len: 6
   • max_len: 6
   • onlist: bc1_onlist.txt (md5: None)
   • regions:
4. linker1

   • region_type: linker1
   • sequence_type: fixed
   • sequence:

     CGACTCACTACAGGG

   • min_len: 15
   • max_len: 15
   • onlist: None
   • regions:
5. bc2

   • region_type: bc2
   • sequence_type: onlist
   • sequence:

     NNNNNN

   • min_len: 6
   • max_len: 6
   • onlist: bc2_onlist.txt (md5: None)
   • regions:
6. linker2

   • region_type: linker2
   • sequence_type: fixed
   • sequence:

     TCGGTGACACGATCG

   • min_len: 15
   • max_len: 15
   • onlist: None
   • regions:
7. bc3

   • region_type: bc3
   • sequence_type: onlist
   • sequence:

     NNNNNN

   • min_len: 6
   • max_len: 6
   • onlist: bc3_onlist.txt (md5: None)
   • regions:
8. umi

   • region_type: umi
   • sequence_type: random
   • sequence:

     NNNNNN

   • min_len: 6
   • max_len: 6
   • onlist: None
   • regions:
9. polyT

   • region_type: polyT
   • sequence_type: random
   • sequence:

     X

   • min_len: 1
   • max_len: 98
   • onlist: None
   • regions:
10. cdna

    • region_type: cdna
    • sequence_type: random
    • sequence:

      X

    • min_len: 1
    • max_len: 98
    • onlist: None
    • regions:
11. ME

    • region_type: ME
    • sequence_type: fixed
    • sequence:

      CTGTCTCTTATACACATCT

    • min_len: 19
    • max_len: 19
    • onlist: None
    • regions:
12. s7

    • region_type: s7
    • sequence_type: fixed
    • sequence:

      CCGAGCCCACGAGAC

    • min_len: 15
    • max_len: 15
    • onlist: None
    • regions:
13. sample_index

    • region_type: sample_index
    • sequence_type: onlist
    • sequence:

      NNNNNNNN

    • min_len: 8
    • max_len: 8
    • onlist: sample_index_onlist.txt (md5: None)
    • regions:
14. illumina_p7

    • region_type: illumina_p7
    • sequence_type: fixed
    • sequence:

      ATCTCGTATGCCGTCTTCTGCTTG

    • min_len: 24
    • max_len: 24
    • onlist: None
    • regions: