PIPseq V2

• https://doi.org/10.1038/s41587-023-01685-z
• PIP-seq is a method that allows us to perform high-throughput droplet single cell RNA-seq without microfluidic device.
• RNA

Final library

PIPseq V2 RNA

AATGATACGGCGACCACCGAGATCTACACGCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGTACGACTCCTCTTTCCCTACACGACGCTCTTCCGATCTNNNNNNNNATGCATCNNNNNNNNCCTCGAGNNNNNNNNNNNNNNNNNNNNXCTGTCTCTTATACACATCTCCGAGCCCACGAGACNNNNNNNNATCTCGTATGCCGTCTTCTGCTTG

1. illumina_p5

   • region_type: illumina_p5
   • sequence_type: fixed
   • sequence:

     AATGATACGGCGACCACCGAGATCTACAC

   • min_len: 29
   • max_len: 29
   • onlist: None
   • regions:
2. linker1

   • region_type: linker
   • sequence_type: fixed
   • sequence:

     GCCTGTCCGCGG

   • min_len: 12
   • max_len: 12
   • onlist: None
   • regions:
3. tso

   • region_type: tso
   • sequence_type: fixed
   • sequence:

     AAGCAGTGGTATCAACGCAGAGT

   • min_len: 23
   • max_len: 23
   • onlist: None
   • regions:
4. linker2

   • region_type: linker
   • sequence_type: fixed
   • sequence:

     ACGACTCC

   • min_len: 8
   • max_len: 8
   • onlist: None
   • regions:
5. truseq_read1

   • region_type: truseq_read1
   • sequence_type: fixed
   • sequence:

     TCTTTCCCTACACGACGCTCTTCCGATCT

   • min_len: 29
   • max_len: 29
   • onlist: None
   • regions:
6. R1.fastq.gz

   • region_type: fastq
   • sequence_type: joined
   • sequence:

     NNNNNNNNATGCATCNNNNNNNNCCTCGAGNNNNNNNNNNNNNNNNNNNN

   • min_len: 50
   • max_len: 50
   • onlist: None
   • regions:
     1. cb1

        • region_type: barcode
        • sequence_type: onlist
        • sequence:

          NNNNNNNN

        • min_len: 8
        • max_len: 8
        • onlist: pip-seq_v2_bc1.tsv (md5: None)
        • regions:
     2. linker3

        • region_type: linker
        • sequence_type: fixed
        • sequence:

          ATGCATC

        • min_len: 7
        • max_len: 7
        • onlist: None
        • regions:
     3. cb2

        • region_type: barcode
        • sequence_type: onlist
        • sequence:

          NNNNNNNN

        • min_len: 8
        • max_len: 8
        • onlist: pip-seq_v2_bc2.tsv (md5: None)
        • regions:
     4. linker4

        • region_type: linker
        • sequence_type: fixed
        • sequence:

          CCTCGAG

        • min_len: 7
        • max_len: 7
        • onlist: None
        • regions:
     5. cb3

        • region_type: barcode
        • sequence_type: onlist
        • sequence:

          NNNNNNNN

        • min_len: 8
        • max_len: 8
        • onlist: pip-seq_v2_bc3.tsv (md5: None)
        • regions:
     6. umi

        • region_type: umi
        • sequence_type: random
        • sequence:

          NNNNNNNNNNNN

        • min_len: 12
        • max_len: 12
        • onlist: None
        • regions:
7. R2.fastq.gz

   • region_type: fastq
   • sequence_type: joined
   • sequence:

     X

   • min_len: 1
   • max_len: 98
   • onlist: None
   • regions:
     1. cDNA

        • region_type: cDNA
        • sequence_type: random
        • sequence:

          X

        • min_len: 1
        • max_len: 98
        • onlist: None
        • regions:
8. nextera_read2

   • region_type: nextera_read2
   • sequence_type: fixed
   • sequence:

     CTGTCTCTTATACACATCTCCGAGCCCACGAGAC

   • min_len: 34
   • max_len: 34
   • onlist: None
   • regions:
     1. ME2

        • region_type: ME2
        • sequence_type: fixed
        • sequence:

          CTGTCTCTTATACACATCT

        • min_len: 19
        • max_len: 19
        • onlist: None
        • regions:
     2. s7

        • region_type: s7
        • sequence_type: fixed
        • sequence:

          CCGAGCCCACGAGAC

        • min_len: 15
        • max_len: 15
        • onlist: None
        • regions:
9. I1.fastq.gz

   • region_type: fastq
   • sequence_type: joined
   • sequence:

     NNNNNNNN

   • min_len: 8
   • max_len: 8
   • onlist: None
   • regions:
     1. index7

        • region_type: index7
        • sequence_type: onlist
        • sequence:

          NNNNNNNN

        • min_len: 8
        • max_len: 8
        • onlist: index7_onlist.txt (md5: None)
        • regions:
10. illumina_p7

    • region_type: illumina_p7
    • sequence_type: fixed
    • sequence:

      ATCTCGTATGCCGTCTTCTGCTTG

    • min_len: 24
    • max_len: 24
    • onlist: None
    • regions: