Quartz-seq

• https://doi.org/10.1186/gb-2013-14-4-r31
• Riken technology for single-cell RNA-seq
• RNA

Final library

RNA

AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTXAGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNATCTCGTATGCCGTCTTCTGCTTG

1. illumina_p5

   • region_type: illumina_p5
   • sequence_type: fixed
   • sequence:

     AATGATACGGCGACCACCGAGATCTACAC

   • min_len: 29
   • max_len: 29
   • onlist: None
   • regions:
2. truseq_read_1

   • region_type: truseq_read_1
   • sequence_type: fixed
   • sequence:

     TCTTTCCCTACACGACGCTCTTCCGATCT

   • min_len: 29
   • max_len: 29
   • onlist: None
   • regions:
3. cDNA

   • region_type: cDNA
   • sequence_type: random
   • sequence:

     X

   • min_len: 1
   • max_len: 98
   • onlist: None
   • regions:
4. truseq_read_2

   • region_type: truseq_read_2
   • sequence_type: fixed
   • sequence:

     AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC

   • min_len: 34
   • max_len: 34
   • onlist: None
   • regions:
5. cell_index

   • region_type: cell_index
   • sequence_type: random
   • sequence:

     NNNNNN

   • min_len: 6
   • max_len: 6
   • onlist: None
   • regions:
6. illumina_p7

   • region_type: illumina_p7
   • sequence_type: fixed
   • sequence:

     ATCTCGTATGCCGTCTTCTGCTTG

   • min_len: 24
   • max_len: 24
   • onlist: None
   • regions: